Scar

Part:BBa_K1969018:Design

Designed by: Yijie Lai   Group: iGEM16_SJTU-BioX-Shanghai   (2016-10-12)


The linker sequence between GAL1 promoter and ADRB2 (with His-tag)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 11
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 11
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 11
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Introduction of the restriction sites for PacI may make the construct more suitable for other elements.


Source

It is designed artificially.

References